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Product Name
GSTA1 antibody
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Description
GSTA1 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human kidney tissue, human liver cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, human kidney tissue. Observed molecular weight by Western-blot: 26kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Glutathione S transferase A1 antibody; GST class alpha member 1 antibody; GST epsilon antibody; GST HA subunit 1 antibody; GST2 antibody; GSTA1 antibody; GSTA1 1 antibody; GTH1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GSTA1 recombinant protein (Accession Number: NM_145740). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:111179(GSTA1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human liver using Catalog No:111179(GSTA1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:111179(GSTA1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using GSTA1 antibody Catalog No:111179 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-GSTA1 (IP:Catalog No:111179, 3ug; Detection:Catalog No:111179 1:500) with mouse liver tissue lysate 4000ug.
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Background
GSTA1 represents glutathione S-transferase alpha 1
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References
- Shirai M, Arakawa S, Miida H. Thioacetamide-induced Hepatocellular Necrosis Is Attenuated in Diet-induced Obese Mice. Journal of toxicologic pathology. 26(2):175-86. 2013.
- Rojanathammanee L, Rakoczy S, Brown-Borg HM. Growth hormone alters the glutathione S-transferase and mitochondrial thioredoxin systems in long-living Ames dwarf mice. The journals of gerontology. Series A, Biological sciences and medical sciences. 69(10):1199-211. 2014.
- Rojanathammanee L, Rakoczy S, Kopchick J, Brown-Borg HM. Effects of insulin-like growth factor 1 on glutathione S-transferases and thioredoxin in growth hormone receptor knockout mice. Age (Dordrecht, Netherlands). 36(4):9687. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"