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Product Name
GSK3B antibody
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Description
GSK3B Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in Ethacrynic acid treated HeLa cells. Positive IHC detected in human testis tissue. Positive WB detected in mouse brain tissue, HeLa cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IF, IHC, FC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
GSK 3 beta antibody; GSK3 beta antibody; GSK3A B antibody; GSK3B antibody; GSK-3B antibody; GSK3B 216P antibody; gsk3beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GSK3B recombinant protein (Accession Number: NM_002093). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111170(GSK3B Antibody) at dilution of 1:1000
IP Result of anti-GSK3B (IP:Catalog No:111170, 4ug; Detection:Catalog No:111170 1:1000) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:111170(GSK3B Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:111170(GSK3B Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using Catalog No:111170(GSK3B Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
1X10^6 HeLa cells were stained with .05ug GSK3B antibody (Catalog No:111170, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.
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Background
GSK3B represents glycogen synthase kinase 3 beta
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