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Product Name
GSDMD antibody
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Description
GSDMD Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human skin tissue. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 50-53 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DF5L antibody; DFNA5L antibody; FKSG10 antibody; FLJ12150 antibody; gasdermin D antibody; GSDMD antibody; GSDMDC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GSDMD recombinant protein (Accession Number: NM_024736). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111166(GSDMD antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human skin using Catalog No:111166(GSDMD antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:111166(GSDMD antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:111166(GSDMD Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GSDMD represents gasdermin D
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References
- He WT, Wan H, Hu L. Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. Cell research. 25(12):1285-98. 2015.
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