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Product Name
GRP94 antibody
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Description
GRP94 Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human lung cancer tissue. Positive IF detected in MCF-7 cells, HEK-293 cells. Positive IP detected in MCF-7 cells. Positive WB detected in A549 cells, HEK-293 cells, K-562 cells, MCF7 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 94 kDa
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Tested applications
ELISA, WB, IHC, FC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ECGP antibody; Endoplasmin antibody; GP96 antibody; gp96 homolog antibody; GRP 94 antibody; GRP94 antibody; HSP90B1 antibody; TRA1 antibody; Tumor rejection antigen 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GRP94 recombinant protein (Accession Number: BC009195). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:111222(HSP90B1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111222(HSP90B1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111222(HSP90B1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-GRP94 (IP:Catalog No:111222, 4ug; Detection:Catalog No:111222 1:500) with MCF-7 cells lysate 4000ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:111222(GRP94 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 MCF-7 cells were stained with 0.2ug GRP94 antibody (Catalog No:111222, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"