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GRP94 antibody

Cat.#: 107306

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Product Information

  • Product Name
    GRP94 antibody
  • Documents
  • Description
    GRP94 Mouse Monoclonal antibody. Positive WB detected in Human heart, human liver tissue, human lung tissue, NIH/3T3 cells. Positive IF detected in Hela cells, HepG2 cells, MCF-7 cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 95kd
  • Tested applications
    ELISA, WB, IF, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    ECGP antibody; GP96 antibody; GRP 94 antibody; GRP94 antibody; HSP90B1 antibody; TRA1 antibody; Tumor rejection antigen 1 antibody
  • Isotype
    Mouse IgM
  • Preparation
    This antibody was obtained by immunization of GRP94 recombinant protein (Accession Number: BC009195). Purification method: Caprylic acid/ammonium sulfate precipitation.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107306(HSP90B1 antibody) at dilution of 1:1000

    human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107306(HSP90B1 antibody) at dilution of 1:1000

    Immunofluorescent analysis of Hela cells, using HSP90B1 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of Hela cells, using HSP90B1 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    1X10^6 HeLa cells were stained with 0.2ug GRP94 antibody (Catalog No:107306, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug GRP94 antibody (Catalog No:107306, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"