GRM4 Polyclonal Antibody

Cat.#: 165291

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Product Information

  • Product Name
    GRM4 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to GRM4
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    GRM4 antibody; GPRC1D antibody; MGLUR4 antibody; mGlu4 antibody; metabotropic glutamate receptor 4 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-290 of human GRM4 (NP_000832.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:100
  • Validations

    Western blot - GRM4 Polyclonal Antibody

    Western blot - GRM4 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using GRM4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

    Immunohistochemistry - GRM4 Polyclonal Antibody

    Immunohistochemistry - GRM4 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat brain using PHF19 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - GRM4 Polyclonal Antibody

    Immunohistochemistry - GRM4 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human breast using PHF19 antibody at dilution of 1:100 (40x lens).

    Immunofluorescence - GRM4 Polyclonal Antibody

    Immunofluorescence - GRM4 Polyclonal Antibody

    Immunofluorescence analysis of MCF-7 cells using GRM4 antibody . Blue: DAPI for nuclear staining.

  • Background
    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"