-
Product Name
GNPAT antibody
- Documents
-
Description
GNPAT Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IP detected in COLO 320 cells. Positive WB detected in HepG2 cells, COLO 320 cells, HeLa cells, human placenta tissue, mouse brain tissue, PC-3 cells, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 65-69 kDa
-
Tested applications
ELISA, IF, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
DAP AT antibody; DAPAT antibody; DHAP AT antibody; DHAPAT antibody; GNPAT antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of GNPAT recombinant protein (Accession Number: NM_014236). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
-
Validations
Immunofluorescent analysis of MCF-7 cells, using GNPAT antibody Catalog No:111007 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111007(GNPAT Antibody) at dilution of 1:600
IP Result of anti-GNPAT (IP:Catalog No:111007, 4ug; Detection:Catalog No:111007 1:500) with COLO 320 cells lysate 2400ug.
-
Background
GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS.(PMID:21990100) This antibody recognize the 69kd GNPAT protein.
-
References
- Itzkovitz B, Jiralerspong S, Nimmo G. Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3. Human mutation. 33(1):189-97. 2012.
- Karnati S, Palaniswamy S, Alam MR, Oruqaj G, Stamme C, Baumgart-Vogt E. C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism. Histochemistry and cell biology. 145(3):287-304. 2016.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"