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Product Name
GNB3 antibody
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Description
GNB3 Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, HepG2 cells. Positive IHC detected in human testis tissue, human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 37 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GNB3 antibody; Transducin beta chain 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GNB3 recombinant protein (Accession Number: NM_002075). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110996(GNB3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:110996(GNB3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:110996(GNB3 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:110996(GNB3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"