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Product Name
GNA13 Polyclonal Antibody
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Description
Polyclonal antibody to GNA13
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
GNA13 antibody; G13 antibody; G protein subunit alpha 13 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human GNA13
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - GNA13 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GNA13 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Western blot - GNA13 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and GNA13 knockout HeLa cells, using GNA13 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - GNA13 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using GNA13 antibody (40x lens).
Immunohistochemistry - GNA13 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using GNA13 antibody (40x lens).
Immunohistochemistry - GNA13 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using GNA13 antibody (40x lens).
Immunohistochemistry - GNA13 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using GNA13 antibody (40x lens).
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Background
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"