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Product Name
GLUD2 antibody
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Description
GLUD2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, A431 cells, HepG2 cells, human testis tissue, mouse liver tissue, mouse testis tissue. Positive IP detected in mouse liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 61-65 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GDH 2 antibody; GDH2 antibody; GLUD2 antibody; GLUDP1 antibody; glutamate dehydrogenase 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GLUD2 recombinant protein (Accession Number: NM_012084). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111075(GLUD2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111075(GLUD2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111075(GLUD2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using GLUD2 antibody Catalog No:111075 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-GLUD2 (IP:Catalog No:111075, 4ug; Detection:Catalog No:111075 1:1000) with mouse liver tissue lysate 4000ug.
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Background
GLUD2 represents glutamate dehydrogenase 2
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