GLAST antibody

GLAST antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

GLAST Rabbit Polyclonal antibody. Positive WB detected in Neuro-2a cells, C6 cells, mouse brain tissue. Positive IHC detected in human brain tissue, mouse brain tissue. Observed molecular weight by Western-blot: 50-55, 90-100 kDa

ELISA, IHC, WB

Human, Mouse, Rat; other species not tested.

EA6 antibody; EAAT1 antibody; GLAST antibody; GLAST 1 antibody; GLAST1 antibody; SLC1A3 antibody

Rabbit IgG

This antibody was obtained by immunization of GLAST recombinant protein (Accession Number: NM_004172). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)