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  • GIT1 Polyclonal Antibody
  • GIT1 Polyclonal Antibody

GIT1 Polyclonal Antibody

Cat.#: 166598

Special Price 251.6 USD

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Product Information

  • Product Name
    GIT1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to GIT1
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    GIT1 antibody; GIT ArfGAP 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:200 - 1:2000
  • Validations

    Western blot - GIT1 Polyclonal Antibody

    Western blot - GIT1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using GIT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

    Western blot - GIT1 Polyclonal Antibody

    Western blot - GIT1 Polyclonal Antibody

    Western blot analysis of extracts from normal (control) and GIT1 knockout HeLa cells, using GIT1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

  • Background
    GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (By similarity).
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"