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Product Name
GEMIN8 antibody
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Description
GEMIN8 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HEK-293 cells. Positive IP detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
FAM51A1 antibody; FLJ20514 antibody; FLJ43072 antibody; Gem assOCiated protein 8 antibody; Gemin 8 antibody; GEMIN8 antibody; Protein FAM51A1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GEMIN8 recombinant protein (Accession Number: XM_017029617). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110941(GEMIN8 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:110941(GEMIN8 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:110941(GEMIN8 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-GEMIN8 (IP:Catalog No:110941, 3ug; Detection:Catalog No:110941 1:400) with HepG2 cells lysate 1720ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:110941(GEMIN8 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"