Product Name

GEFT-Specific antibody

Documents

Description

GEFT-Specific Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue, HEK-293 cells, mouse heart tissue, mouse liver tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human pancreas tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 64 kDa

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

GEFT antibody; p63RhoGEF antibody; RhoA/RAC/CDC42 exchange factor antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Peptide (Accession Number: NM_182947). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

Validations

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:110935(GEFT-Specific antibody) at dilution of 1:1500

IP Result of anti-GEFT-Specific (IP:Catalog No:110935, 4ug; Detection:Catalog No:110935 1:1500) with mouse brain tissue lysate 8000ug.

Immunohistochemistry of paraffin-embedded human pancreas slide using Catalog No:110935(GEFT-Specific Antibody) at dilution of 1:100

Background

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis.

References

Bryan BA, Mitchell DC, Zhao L. Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT. Molecular and cellular biology. 25(24):11089-101. 2005.
Wuertz CM, Lorincz A, Vettel C, Thomas MA, Wieland T, Lutz S. p63RhoGEF--a key mediator of angiotensin II-dependent signaling and processes in vascular smooth muscle cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 24(12):4865-76. 2010.
Mitchell DC, Bryan BA, Liu L. GEFT, A Rho family guanine nucleotide exchange factor, regulates lens differentiation through a Rac1-mediated mechanism. Current molecular medicine. 11(6):465-80. 2011.
Momotani K, Artamonov MV, Utepbergenov D, Derewenda U, Derewenda ZS, Somlyo AV. p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility. Circulation research. 109(9):993-1002. 2011.
Kant S, Swat W, Zhang S. TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway. Genes & development. 25(19):2069-78. 2011.
Hayashi A, Hiatari R, Tsuji T, Ohashi K, Mizuno K. p63RhoGEF-mediated formation of a single polarized lamellipodium is required for chemotactic migration in breast carcinoma cells. FEBS letters. 587(6):698-705. 2013.
Calò LA, Davis PA, Pagnin E. Increased level of p63RhoGEF and RhoA/Rho kinase activity in hypertensive patients. Journal of hypertension. 32(2):331-8. 2014.
Artamonov MV, Momotani K, Stevenson A. Agonist-induced Ca2+ sensitization in smooth muscle: redundancy of Rho guanine nucleotide exchange factors (RhoGEFs) and response kinetics, a caged compound study. The Journal of biological chemistry. 288(47):34030-40. 2013.

GEFT-Specific antibody

Product Information

Related Products / Services