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Product Name
GDI2 antibody
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Description
GDI2 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IHC detected in human skin. Observed molecular weight by Western-blot: 46-50kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human; other species not tested.
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Alternative names
GDI2 antibody; Rab GDI beta antibody; FLJ37352 antibody; RABGDIB antibody; FLJ16452 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001494). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110928(GDI2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human skin using Catalog No:110928(GDI2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:110928(GDI2 antibody) at dilution of 1:50 (under 40x lens)
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Background
GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). The antibody is specific to GDI2. And it has no cross reaction to GDI1.
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