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Product Name
GDI1 antibody
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Description
GDI1 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, human brain tissue. Positive IHC detected in human brain tissue, human kidney tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ41411 antibody; GDI 1 antibody; GDI1 antibody; GDIL antibody; GDP dissOCiation inhibitor 1 antibody; MRX41 antibody; MRX48 antibody; Oligophrenin 2 antibody; OPHN2 antibody; Protein XAP 4 antibody; Rab GDI alpha antibody; RABGD1A antibody; RABGDIA antibody; XAP 4 antibody; XAP4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GDI1 recombinant protein (Accession Number: NM_001493). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110925(GDI1 Antibody) at dilution of 1:1000
IP Result of anti-GDI1 (IP:Catalog No:110925, 4ug; Detection:Catalog No:110925 1:800) with mouse brain tissue lysate 4000ug.
Immunohistochemical of paraffin-embedded human brain using Catalog No:110925(GDI1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:110925(GDI1 antibody) at dilution of 1:50 (under 40x lens)
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Background
GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55kda protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034) This antibody can bind both GDIs for the close sequences.
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References
- Li X, Sapp E, Chase K. Disruption of Rab11 activity in a knock-in mouse model of Huntington's disease. Neurobiology of disease. 36(2):374-83. 2009.
- Kimura A, Kanoh Y, Sakurai T. Antibodies in patients with neuropsychiatric systemic lupus erythematosus. Neurology. 74(17):1372-9. 2010.
- English JA, Dicker P, Föcking M, Dunn MJ, Cotter DR. 2-D DIGE analysis implicates cytoskeletal abnormalities in psychiatric disease. Proteomics. 9(12):3368-82. 2009.
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