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Product Name
GCOM1 antibody
- Documents
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Description
GCOM1 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue. Positive IHC detected in human liver tissue. Positive IF detected in HepG2 cells, Hela cells. Observed molecular weight by Western-blot: 64kd,44-54kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ30973 antibody; Gcom antibody; GCOM1 antibody; Gcom2 antibody; GRINL1A antibody; GRINL1A combined protein antibody; GRINL1A complex lOCus antibody; GRINL1A upstream protein antibody; Gup antibody; Gup1 antibody; Gup2 antibody; MYOZAP antibody; MYZAP antibody; POLR2M antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GCOM1 recombinant protein (Accession Number: NM_001018096). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110913(GCOM1 antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human liver using Catalog No:110913(GCOM1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:110913(GCOM1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using GCOM1 antibody Catalog No:110913 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
GCOM1, also named as Myozap, Gup and Gcom, belongs to the GCOM1 family. It plays a role in cellular signaling via Rho-related GTP-binding proteins and subsequent activation of transcription factor SRF. GCOM1 has 11 isoforms with MW 44-54kd and 64kd.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"