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gamma Tubulin antibody

Cat.#: 110859

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Product Information

  • Product Name
    gamma Tubulin antibody
  • Documents
  • Description
    gamma Tubulin Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE cells , MDCK cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 50-55 kDa
  • Tested applications
    ELISA, IF, WB, IP
  • Species reactivity
    Human,Mouse,Rat, Dog; other species not tested.
  • Alternative names
    Gamma 1 tubulin antibody; gamma Tubulin antibody; GCP 1 antibody; TUBG antibody; TUBG1 antibody; TUBGCP1 antibody; Tubulin gamma 1 chain antibody; tubulin antibody; gamma 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of gamma Tubulin recombinant protein (Accession Number: NM_001070). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:1000-1:10000

    IF: N/A

  • Validations

    IF results of gamma tubulin antibody (Catalog No:110859, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.

    IF results of gamma tubulin antibody (Catalog No:110859, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.

    IF results of gamma tubulin antibody (Catalog No:110859, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.

    IF results of gamma tubulin antibody (Catalog No:110859, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.

    IF results of gamma tubulin antibody (Catalog No:110859, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.

    IF results of gamma tubulin antibody (Catalog No:110859, 1:100) with hTERT-RPE cells by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Cells were fixed in ice-cold methanol.

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110859(tubulin-gamma Antibody) at dilution of 1:600

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110859(tubulin-gamma Antibody) at dilution of 1:600

    IP Result of anti-tubulin-gamma (IP:Catalog No:110859, 4ug; Detection:Catalog No:110859 1:2000) with HeLa cells lysate 1600ug.

    IP Result of anti-tubulin-gamma (IP:Catalog No:110859, 4ug; Detection:Catalog No:110859 1:2000) with HeLa cells lysate 1600ug.

    Immunofluorescent analysis of MDCK cells using Catalog No:110859(tubulin-gamma Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of MDCK cells using Catalog No:110859(tubulin-gamma Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"