Product Name

Gamma cystathionase antibody

Documents

Description

Gamma cystathionase Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in mouse liver tissue, HepG2 cells, mouse heart tissue, rat heart tissue, rat liver tissue. Positive FC detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue, human kidney tissue. Observed molecular weight by Western-blot: 43-45 kDa,66-70 kDa

Tested applications

ELISA, IHC, IP, WB, FC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

CSE antibody; CTH antibody; Cystathionine gamma lyase antibody; Gamma cystathionase antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Gamma cystathionase recombinant protein (Accession Number: NM_001902). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

Validations

WB result of CTH antibody (Catalog No:110858, 1:500) with si-control and si-CTH transfected HepG2 cell.

mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110858(CSE antibody) at dilution of 1:1000

IP Result of anti-CSE (IP:Catalog No:110858, 3ug; Detection:Catalog No:110858 1:1000) with HEK-293 cells lysate 2700ug.

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110858(CSE antibody) at dilution of 1:50 (under 10x lens)

1X10^6 MCF-7 cells were stained with 0.2ug CSE antibody (Catalog No:110858, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Background

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation.It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.

References

Altaany Z, Yang G, Wang R. Crosstalk between hydrogen sulfide and nitric oxide in endothelial cells. Journal of cellular and molecular medicine. 17(7):879-88. 2013.
Pan Y, Zhou C, Yuan D, Zhang J, Shao C. Radiation Exposure Promotes Hepatocarcinoma Cell Invasion through Epithelial Mesenchymal Transition Mediated by H2S/CSE Pathway. Radiation research. 185(1):96-105. 2016.
Flannigan KL, Agbor TA, Motta JP. Proresolution effects of hydrogen sulfide during colitis are mediated through hypoxia-inducible factor-1α. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(4):1591-602. 2015.
Zhao K, Ju Y, Li S, Altaany Z, Wang R, Yang G. S-sulfhydration of MEK1 leads to PARP-1 activation and DNA damage repair. EMBO reports. 15(7):792-800. 2014.
Altaany Z, Ju Y, Yang G, Wang R. The coordination of S-sulfhydration, S-nitrosylation, and phosphorylation of endothelial nitric oxide synthase by hydrogen sulfide. Science signaling. 7(342):ra87. 2014.
Salloum FN, Sturz GR, Yin C. Beetroot juice reduces infarct size and improves cardiac function following ischemia-reperfusion injury: Possible involvement of endogenous H2S. Experimental biology and medicine (Maywood, N.J.). 240(5):669-81. 2015.
Bos EM, Wang R, Snijder PM. Cystathionine γ-lyase protects against renal ischemia/reperfusion by modulating oxidative stress. Journal of the American Society of Nephrology : JASN. 24(5):759-70. 2013.
Asimakopoulou A, Panopoulos P, Chasapis CT. Selectivity of commonly used pharmacological inhibitors for cystathionine β synthase (CBS) and cystathionine γ lyase (CSE). British journal of pharmacology. 169(4):922-32. 2013.

Gamma cystathionase antibody

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