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Product Name
GALE antibody
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Description
GALE Rabbit Polyclonal antibody. Positive IHC detected in human malignant melanoma tissue. Positive WB detected in A375 cells, HeLa cells, MCF7 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 38 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ95174 antibody; FLJ97302 antibody; Galactowaldenase antibody; GALE antibody; SDR1E1 antibody; UDP glucose 4 epimerase antibody; UDP-galactose-4-epimerase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GALE recombinant protein (Accession Number: NM_000403). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:110835(GALE antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human malignant melanoma using Catalog No:110835(GALE antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human malignant melanoma using Catalog No:110835(GALE antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-GALE (IP:Catalog No:110835, 4ug; Detection:Catalog No:110835 1:300) with HeLa cells lysate 1080ug.
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Background
GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).
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References
- Wen Y, Qin J, Deng Y, Wang H, Magdalou J, Chen L. The critical role of UDP-galactose-4-epimerase in osteoarthritis: modulating proteoglycans synthesis of the articular chondrocytes. Biochemical and biophysical research communications. 452(4):906-11. 2014.
- Wen Y, Li J, Tan Y. Angelica Sinensis polysaccharides stimulated UDP-sugar synthase genes through promoting gene expression of IGF-1 and IGF1R in chondrocytes: promoting anti-osteoarthritic activity. PloS one. 9(9):e107024. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"