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Product Name
Galc antibody
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Description
Galc Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer. Positive WB detected in A375 cells, human brain tissue, human placenta tissue. Observed molecular weight by Western-blot: 50-55kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
Galc antibody; 2310068B06Rik antibody; twitcher antibody; AW413532 antibody; AW212969 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Galc recombinant protein (Accession Number: BC086671). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:110831(Galc antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110831(Galc antibody) at dilution of 1:50 (under 10x lens)
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Background
GALC, also named as Galactosylceramidase , belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is an enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits. (PMID:8634707 )
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