-
Product Name
GAGE2D antibody
- Documents
-
Description
GAGE2D Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue, human testis tissue. Positive WB detected in K-562 cells, HeLa cells, human testis tissue. Observed molecular weight by Western-blot: 18-22kd
-
Tested applications
ELISA, WB, IF, IHC
-
Species reactivity
Human; other species not tested.
-
Alternative names
Cancer/testis antigen 4.8 antibody; CT4.8 antibody; G antigen 2D antibody; G antigen 8 antibody; GAGE 8 antibody; GAGE2D antibody; GAGE8 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of GAGE2D recombinant protein (Accession Number: NM_001127200). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:110825(GAGE2D antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using GAGE2D antibody Catalog No:110825 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110825(GAGE2D Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110825(GAGE2D Antibody) at dilution of 1:50 (under 40x lens)
-
Background
GAGE2D, also named as GAGE8 and CT4.8, is expressed in testis and tumor tissues.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"