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Product Name
Mouse Cyclin E1/CCNE1 (His & GST Tag) recombinant protein
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Description
Essential for the control of the cell cycle at the G1/S (start) transition.
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Protein name
G1/S-specific cyclin-E1
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Protein short names
CCNE; CYCE1; AW538188; CCNE1; CYCLIN E1
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Uniprot ID
Q61457
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Gene Name
Ccne1; Ccne
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse CCNE1 (AAH62152.1) (Met1-Glu408) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse CCNE1/GST chimera consists of 645 amino acids and has a calculated molecular mass of 74.8 kDa. The recombinant protein migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized mouse CCNE1 (Cat:501490) at 10 μg/ml (100 μl/well) can bind biotinylated human CDK4 (Cat:501272), The EC50 of biotinylated human CDK4 (Cat:501272) is 48.0-114.0 ng/ml. -
Validations
Mouse CCNE1 / Cyclin-E1 Protein (His & GST Tag) SDS-PAGE
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