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Product Name
Anti-Phospho-Cyclin E1 (Thr395) Rabbit antibody
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Description
Phospho-Cyclin E1 (Thr395) Rabbit polyclonal antibody
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Tested applications
WB
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of threonine 395 (L-L-T(p)-P-P)derived from Human Cyclin E1
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/1000
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Validations
Western blot analysis of extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1 (phospho-Thr395) Antibody .
Western blot detection of Cyclin E1(phospho-Thr395) in Hela cells untreated or treated with UV using Cyclin E1(phospho-Thr395) Rabbit pAb (dilution 1:500, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:48kDa.Observed band size:48kDa.
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Background
Swiss-Prot Acc.P24864.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"