-
Product Name
FKBP8 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to FKBP8
-
Tested applications
WB, IF, IP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
FKBP8 antibody; FKBP38 antibody; FKBPr38 antibody; FK506 binding protein 8 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-270 of human FKBP8 (NP_036313.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200 -
Validations
Western blot - FKBP8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FKBP8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunofluorescence - FKBP8 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using FKBP8 antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - FKBP8 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FKBP8 antibody . Western blot was performed from the immunoprecipitate using FKBP8 antibody at a dilition of 1:1000.
-
Background
Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"