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Product Name
FHL3 antibody
- Documents
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Description
FHL3 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells, mouse ovary tissue. Observed molecular weight by Western-blot: 34kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FHL 3 antibody; FHL3 antibody; four and a half LIM domains 3 antibody; Skeletal muscle LIM protein 2 antibody; SLIM 2 antibody; SLIM2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FHL3 recombinant protein (Accession Number: NM_004468). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:110651(FHL3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110651(FHL3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110651(FHL3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-FHL3 (IP:Catalog No:110651, 4ug; Detection:Catalog No:110651 1:500) with K-562 cells lysate 1200ug.
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Background
Four and a half LIM domain (FHL) protein 3 is a member of the FHL protein family that has roles in the regulation of signal transduction, survival, cell adhesion, and mobility. It also involves in the development and progression of liver cancer. The FHL proteins have been shown to regulate a variety of transcription factors, including SMAD proteins, β-catenin, SRF, AP-1, NFAT, FOXO1, MyoD, and the androgen receptor. Beside, the FHL proteins may involve in muscle growth and differentiation. Recent research revealed FHL3 was a PCBP2 target, also involved in the growth and induced apoptosis of glioma cell (23585479). This antibody recognizes specifically the 34kd FHL3 protein.
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References
- Xia W, Fu W, Cai L. Identification and characterization of FHL3 as a novel angiogenin-binding partner. Gene. 504(2):233-7. 2012.
- Lin J, Qin X, Zhu Z. FHL family members suppress vascular endothelial growth factor expression through blockade of dimerization of HIF1α and HIF1β. IUBMB life. 64(11):921-30. 2012.
- Han W, Xin Z, Zhao Z. RNA-binding protein PCBP2 modulates glioma growth by regulating FHL3. The Journal of clinical investigation. 123(5):2103-18. 2013.
- McCabe Á, Hashimoto K, Hall WW, Sheehy N. The four and a half LIM family members are novel interactants of the human T-cell leukemia virus type 1 Tax oncoprotein. Journal of virology. 87(13):7435-44. 2013.
- Cai X, Wang J, Huang X. Identification and characterization of MT-1X as a novel FHL3-binding partner. PloS one. 9(4):e93723. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"