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Product Name
FHL2 Polyclonal Antibody
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Description
Polyclonal antibody to FHL2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
FHL2 antibody; AAG11 antibody; DRAL antibody; FHL-2 antibody; SLIM-3 antibody; SLIM3 antibody; four and a half LIM domains 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-279 of human FHL2 (NP_001034581.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - FHL2 Polyclonal Antibody
Western blot analysis of extracts of ES-2 cells, using FHL2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .
Immunohistochemistry - FHL2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using FHL2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - FHL2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using FHL2 antibody at dilution of 1:100 (40x lens).
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Background
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"