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Product Name
FHL2 antibody
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Description
FHL2 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human heart tissue. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in mouse ovary tissue, HeLa cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AAG11 antibody; DRAL antibody; FHL 2 antibody; FHL2 antibody; four and a half LIM domains 2 antibody; LIM domain protein DRAL antibody; Skeletal muscle LIM protein 3 antibody; SLIM 3 antibody; SLIM3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FHL2 recombinant protein (Accession Number: NM_201557). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
mouse ovary tissue were subjected to SDS PAGE followed by western blot with Catalog No:110650(FHL2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human heart using Catalog No:110650(FHL2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:110650(FHL2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-FHL2 (IP:Catalog No:110650, 4ug; Detection:Catalog No:110650 1:1000) with mouse skeletal muscle tissue lysate 2200ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:110650(FHL2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FHL2 represents four and a half LIM domains 2
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