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Product Name
FGFR1 antibody
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Description
FGFR1 Mouse Monoclonal antibody. Positive FC detected in K-562 cells. Positive IHC detected in human breast cancer tissue. Positive WB detected in Y79 cells, 3T3-L1 cells, K-562 cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
bFGF R 1 antibody; BFGFR antibody; CD331 antibody; CEK antibody; FGFBR antibody; FGFR 1 antibody; FGFR1 antibody; FLG antibody; FLJ99988 antibody; FLT 2 antibody; FLT2 antibody; Fms like tyrosine kinase 2 antibody; HBGFR antibody; KAL2 antibody; N SAM antibody; OGD antibody; Proto oncogene c Fgr antibody
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Isotype
Mouse IgG2c
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Preparation
This antibody was obtained by immunization of FGFR1 recombinant protein (Accession Number: NM_015850). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:107209(FGFR1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107209(FGFR1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107209(FGFR1 Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 K-562 cells were stained with 0.2ug FGFR1 antibody (Catalog No:107209, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
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Background
Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processed including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome. Alternatively spliced variants which encode different protein isoforms have been described.
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References
- Wang T, Zhang Y, Wang HD. Alpaca fiber growth is mediated by microRNA let-7b via down-regulation of target gene FGF5. Genetics and molecular research : GMR. 14(4):13754-63. 2015.
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