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Product Name
FER antibody
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Description
FER Rabbit Polyclonal antibody. Positive FC detected in Jurkat cells. Positive IHC detected in human colon cancer tissue, human colon tissue, human kidney tissue. Positive IF detected in MCF-7 cells. Positive WB detected in NIH/3T3 cells, A431 cells, HeLa cells, MCF-7 cells. Observed molecular weight by Western-blot: 95 kDa
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FER antibody; p94 Fer antibody; Proto oncogene c Fer antibody; TYK3 antibody; Tyrosine kinase 3 antibody; Tyrosine protein kinase Fer antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FER recombinant protein (Accession Number: BC017060). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110619(FER Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:110619(FER Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:110619(FER Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:110619(FER Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 Jurkat cells were stained with 0.5ug FER antibody (Catalog No:110619, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"