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Product Name
FBXW7 Polyclonal Antibody
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Description
Polyclonal antibody to FBXW7
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
FBXW7 antibody; AGO antibody; CDC4 antibody; FBW6 antibody; FBW7 antibody; FBX30 antibody; FBXO30 antibody; FBXW6 antibody; SEL-10 antibody; SEL10 antibody; hAgo antibody; hCdc4 antibody; F-box/WD repeat-containing protein 7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human FBXW7
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - FBXW7 Polyclonal Antibody
Western blot analysis of extracts of mouse intestine, using FBXW7 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1, and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation. SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (By similarity). Involved in bone homeostasis and negative regulation of osteoclast differentiation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"