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Product Name
FBXO5 antibody
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Description
FBXO5 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human placenta tissue, human tonsillitis tissue. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells, human ovary tissue, K-562 cells, mouse kidney tissue, rat liver tissue. Observed molecular weight by Western-blot: 56 kDa, 45 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Early mitotic inhibitor 1 antibody; EMI1 antibody; F box only protein 5 antibody; F box protein 5 antibody; FBX5 antibody; FBXO5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FBXO5 recombinant protein (Accession Number: NM_012177). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110556(FBXO5 antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:110556(FBXO5 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:110556(FBXO5 Antibody) at dilution of 1:50
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Background
FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.
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