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Product Name
FBXO32 antibody
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Description
FBXO32 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human skeletal muscle tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse heart tissue, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Atrogin 1 antibody; F box only protein 32 antibody; F box protein 32 antibody; Fbx32 antibody; FBXO32 antibody; FLJ32424 antibody; MAFbx antibody; Muscle atrophy F box protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FBXO32 recombinant protein (Accession Number: NM_148177). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:110553(FBXO32 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:110553(FBXO32 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:110553(FBXO32 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:110553(FBXO32 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FBXO32 (F box only protein 32), also known as Atrogin 1 or MAFbx, is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif F-box. This protein is an E3 ubiquitin ligase that is markedly up-regulated in muscle atrophy. FBXO32 is thus a potential drug target for the treatment of muscle atrophy. Some data support that FBXO32 may play an important role in tumorigenesis. Recent study reveal that FBXO32 targets the oncogenic protein c-Myc for ubiquitination and degradation through the proteasome pathway.
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