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Product Name
FBXL14 antibody
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Description
FBXL14 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue. Positive WB detected in HepG2 cells. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
F box/LRR repeat protein 14 antibody; Fbl14 antibody; FBXL14 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FBXL14 recombinant protein (Accession Number: NM_152441). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110542(FBXL14 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human liver using Catalog No:110542(FBXL14 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:110542(FBXL14 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:110542(FBXL14 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FBXL14(F-box/LRR-repeat protein 14) is also named as FBL14 and belongs to the the F-box protein family,which are characterized by an approximately 40-amino acid F-box motif. It is part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase complex. SNAIL1 is an unstable transcription factor that is phosphorylated by GSK3-beta in the nucleus, which triggers its nuclear export and subsequent ubiquitination and proteasomal degradation. FBXL14 is directed ubiquitination and degradation of SNAIL1 in human and mouse cell lines(PMID:19955572).
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References
- Liu Y, Lear T, Zhao Y. F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7. Cell death & disease. 6:e1630. 2015.
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