FBLIM1 antibody

FBLIM1 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

FBLIM1 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive WB detected in HepG2 cells, HEK-293 cells, HeLa cells, human heart tissue, K-562 cells. Observed molecular weight by Western-blot: 45-48 kDa

ELISA, WB, IHC

Human,Mouse,Rat; other species not tested.

CAL antibody; DKFZp434G171 antibody; FBLIM1 antibody; FBLP 1 antibody; FBLP1 antibody; filamin binding LIM protein 1 antibody; MIG2 interacting protein antibody; Migfilin antibody; RP11 169K16.5 antibody

Rabbit IgG

This antibody was obtained by immunization of FBLIM1 recombinant protein (Accession Number: XM_047423104). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

FBLIM1, also known as CAL, Mig-2-interacting protein or Migfilin, is a cytoplasmic protein that belongs to the LIM superfamily. FBLIM1 is a protein found in cell-cell and cell-ECM connections where it co-localizes with FLNA/C and FLNB. FBLIM1 was found to bind directly to FLNA/C and to be an important regulator of cell shape and motility. FBLIM1 exerts its influence on cellular functions by interacting with various binding partners; FLN via its N- terminal domain, VASP and Src via its proline-rich region, and kindlin-2 and the cardiac transcription factor, CSX/NKX2-5 via its C-terminal LIM domains. Three isoforms exist for FBLIM1 due to alternative splicing events, namely FBLP-1A, FBLP-1 and FBLP-1B. FBLIM1 serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. It is associated with actin stress fiber at cell-ECM focal adhesion sites. FBLP-1A and FBLP-1B are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. FBLP-1 is localized at actin stress fibers. FBLIM1 is implicated in cell shape modulation (spreading) and motility. FBLIM1 participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. It may also regulate the assembly of filamin-containing signaling complexes that control actin assembly. In addition, FBLIM1 is capable of translocating to the nucleus and regulating gene expression. This antibody is a rabbit polyclonal antibody raised against full length human FBLIM1 antigen.

• Bandyopadhyay A, Rothschild G, Kim S, Calderwood DA, Raghavan S. Functional differences between kindlin-1 and kindlin-2 in keratinocytes. Journal of cell science. 125(Pt 9):2172-84. 2012.