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FASTKD2 antibody

Cat.#: 110530

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Product Information

  • Product Name
    FASTKD2 antibody
  • Documents
  • Description
    FASTKD2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, mouse heart tissue, transfected cells. Positive IP detected in mouse heart tissue. Positive IF detected in COS7 cells, U2OS cells. Observed molecular weight by Western-blot: 73-75 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FAST kinase domains 2 antibody; FASTKD2 antibody; KIAA0971 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of FASTKD2 recombinant protein (Accession Number: NM_001136193). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:1000

    IF: 1:20-1:200

  • Validations

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110530(FASTKD2 antibody) at dilution of 1:1000

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110530(FASTKD2 antibody) at dilution of 1:1000

    IF result of anti-FASTKD2 (Catalog No:110530) with COS7 cells.

    IF result of anti-FASTKD2 (Catalog No:110530) with COS7 cells.

    IP Result of anti-FASTKD2 (IP:Catalog No:110530, 4ug; Detection:Catalog No:110530 1:300) with mouse heart tissue lysate 4000ug.

    IP Result of anti-FASTKD2 (IP:Catalog No:110530, 4ug; Detection:Catalog No:110530 1:300) with mouse heart tissue lysate 4000ug.

  • Background
    FASTKD2(FAST kinase domain-containing protein 2) is also named as KIAA0971 and belongs to the FAST kinase family.A potential cleavage site of the 79 kDa FASTKD2 precursor protein by the mitochondrial matrix protease (MMP) is present between M52 and Q53 and predicts the mature imported protein from the FASTKD2 precursor to be composed of 642 amino acid residues for a MW of approximately 73 kDa(PMID: 18771761) and the clinical and MRI findings of the FASTKD2-associated disease are rather unusual for mitochondrial disorders.
  • References
    • Antonicka H, Shoubridge EA. Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis. Cell reports. 2015.
    • Popow J, Alleaume AM, Curk T, Schwarzl T, Sauer S, Hentze MW. FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation. RNA (New York, N.Y.). 21(11):1873-84. 2015.
    • Jourdain AA, Koppen M, Rodley CD. A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function. Cell reports. 10(7):1110-21. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"