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Product Name
FANCD2 Polyclonal Antibody
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Description
Polyclonal antibody to FANCD2
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
FANCD2 antibody; FA-D2 antibody; FA4 antibody; FACD antibody; FAD antibody; FAD2 antibody; FANCD antibody; Fanconi anemia group D2 protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human FANCD2 (NP_149075.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - FANCD2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FANCD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - FANCD2 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using FANCD2 antibody . Blue: DAPI for nuclear staining.
Immunofluorescence - FANCD2 Polyclonal Antibody
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using FANCD2 antibody . Greenuff1aGFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168 can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
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Background
Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"