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Product Name
FA2H antibody
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Description
FA2H Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, HeLa cells, human stomach tissue, mouse colon tissue, SGC-7901 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 38-43kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FA2H antibody; FAAH antibody; FAH1 antibody; fatty acid 2 hydroxylase antibody; Fatty acid alpha hydroxylase antibody; FAXDC1 antibody; FLJ25287 antibody; SCS7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FA2H recombinant protein (Accession Number: NM_024306). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110434(FA2H antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110434(FA2H antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110434(FA2H antibody) at dilution of 1:50 (under 40x lens)
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Background
FA2H(Fatty acid 2-hydroxylase) required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. The deduced 372-amino acid protein has a calculated molecular mass of 42.8 kD .Both mouse and human FA2H have a C-terminal endoplasmic reticulum (ER) retention motif(PMID: 15658937).
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References
- Baguley TD, Xu HC, Chatterjee M, Nairn AC, Lombroso PJ, Ellman JA. Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase. Journal of medicinal chemistry. 56(19):7636-50. 2013.
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