-
Product Name
EXOSC2 antibody
- Documents
-
Description
EXOSC2 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, Jurkat cells, MCF7 cells. Positive IP detected in HeLa cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human skin cancer tissue. Observed molecular weight by Western-blot: 30-33 kDa
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
EXOSC2 antibody; exosome component 2 antibody; hRrp4p antibody; RRP4 antibody; Rrp4p antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of EXOSC2 recombinant protein (Accession Number: NM_014285). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110509(EXOSC2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:110509(EXOSC2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using EXOSC2 antibody Catalog No:110509 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-EXOSC2 (IP:Catalog No:110509, 4ug; Detection:Catalog No:110509 1:1000) with HeLa cells lysate 1080ug.
-
Background
In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"