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Product Name
Human EIF-5A/EIF5 (GST Tag) recombinant protein
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Description
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
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Protein name
Eukaryotic translation initiation factor 5
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Protein short names
D12ERTD549E; EIF-5; EIF-5A; 2810011H21RIK
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Uniprot ID
P55010
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Gene Name
EIF5
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the mature form of human EIF5 (P55010) (Met1-Asp150) was fused with the GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human EIF5 /GST chimera consists of 384 amino acids and has a predicted molecular mass of 44.1 kDa. It migrates as an approximately 38-43 KDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
Human EIF-5A / EIF5 Protein (GST Tag) SDS-PAGE
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