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Product Name
ERO1LB antibody
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Description
ERO1LB Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human placenta tissue. Positive IHC detected in human pancreas cancer tissue, human oesophagus tissue, human small intestine tissue. Observed molecular weight by Western-blot: 40~50kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ERO1 L beta antibody; ERO1 like beta (S. cerevisiae) antibody; ERO1 like protein beta antibody; ERO1LB antibody; Oxidoreductin 1 L beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ERO1LB recombinant protein (Accession Number: NM_019891). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110371(ERO1LB antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110371(ERO1LB antibody) at dilution of 1:50 (under 40x lens)
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Background
Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Mammals express two related Ero proteins, Ero1L alpha and Ero1L beta. The two homologous proteins can form homodimers and mixed heterodimers. ERO1-Lα and -β are highly expressed in organs with a high demand for secretion, consistent with their roles in oxidative folding of ER proteins.
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References
- Khoo C, Yang J, Rajpal G. Endoplasmic reticulum oxidoreductin-1-like β (ERO1lβ) regulates susceptibility to endoplasmic reticulum stress and is induced by insulin flux in β-cells. Endocrinology. 152(7):2599-608. 2011.
- Rajpal G, Schuiki I, Liu M, Volchuk A, Arvan P. Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells. The Journal of biological chemistry. 287(1):43-7. 2012.
- Galligan JJ, Smathers RL, Shearn CT. Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease. Journal of toxicology. 2012:207594. 2012.
- Okamoto Y, Pehlivan D, Wiszniewski W. Curcumin facilitates a transitory cellular stress response in Trembler-J mice. Human molecular genetics. 22(23):4698-705. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"