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ERO1L antibody

Cat.#: 110369

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Product Information

  • Product Name
    ERO1L antibody
  • Documents
  • Description
    ERO1L Rabbit Polyclonal antibody. Positive WB detected in mouse ovary tissue, A431 cells. Positive IP detected in L02 cells. Positive FC detected in HeLa cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 54 kDa
  • Tested applications
    ELISA, IHC, WB, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ERO1 alpha antibody; ERO1 L antibody; ERO1 L alpha antibody; ERO1 like (S. cerevisiae) antibody; ERO1 like protein alpha antibody; ERO1L antibody; Oxidoreductin 1 L alpha antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ERO1L recombinant protein (Accession Number: NM_014584). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with Catalog No:110369(ERO1L antibody) at dilution of 1:100

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with Catalog No:110369(ERO1L antibody) at dilution of 1:100

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110369(ERO1L antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110369(ERO1L antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-ERO1L (IP:Catalog No:110369, 4ug; Detection:Catalog No:110369 1:300) with L02 cells lysate 1400ug.

    IP Result of anti-ERO1L (IP:Catalog No:110369, 4ug; Detection:Catalog No:110369 1:300) with L02 cells lysate 1400ug.

    1X10^6 HeLa cells were stained with 0.2ug ERO1L antibody (Catalog No:110369, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug ERO1L antibody (Catalog No:110369, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. This antibody has no cross reaction to ERO1L.
  • References
    • Galligan JJ, Smathers RL, Shearn CT. Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease. Journal of toxicology. 2012:207594. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"