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Product Name
ERO1A Polyclonal Antibody
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Description
Polyclonal antibody to ERO1A
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
ERO1A antibody; ERO1-L antibody; ERO1-L-alpha antibody; ERO1-alpha antibody; ERO1L antibody; ERO1LA antibody; Ero1alpha antibody; ERO1-like protein alpha antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human ERO1A
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - ERO1A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ERO1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"