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Product Name
ERGIC1 antibody
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Description
ERGIC1 Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, human liver tissue, L02 cells, mouse liver tissue. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, IHC, IP, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ERGIC 32 antibody; ERGIC1 antibody; ERGIC32 antibody; FLJ39864 antibody; KIAA1181 antibody; NET24 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ERGIC1 recombinant protein (Accession Number: NM_001031711). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110356(ERGIC1 Antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110356(ERGIC1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110356(ERGIC1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ERGIC1 (IP:Catalog No:110356, 3ug; Detection:Catalog No:110356 1:500) with HepG2 cells lysate 1720ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:110356(ERGIC1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The function of ERGIC1 remains largely unknown. ERGIC1 was highly expressed in most primary prostate tumors. It is an intriguing potential drug target especially for the ERG oncogene expressing tumors (PMID: 22761906). The MW of this protein is 32 kDa, and this antibody specially recognises the 32 kDa protein.
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References
- Tan SM, Kirchner R, Jin J. Sequencing of captive target transcripts identifies the network of regulated genes and functions of primate-specific miR-522. Cell reports. 8(4):1225-39. 2014.
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