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Product Name
ERAL1 antibody
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Description
ERAL1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, K-562 cells. Positive IP detected in Jurkat cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 50-52 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CEGA antibody; Conserved ERA like GTPase antibody; ERA antibody; Era G protein like 1 (E. coli) antibody; ERA W antibody; ERAL1 antibody; ERAL1A antibody; hERA antibody; HERA A antibody; HERA B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ERAL1 recombinant protein (Accession Number: NM_005702). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:110399(ERAL1 Antibody) at dilution of 1:1000
IP Result of anti-ERAL1 (IP:Catalog No:110399, 4ug; Detection:Catalog No:110399 1:500) with Jurkat cells lysate 4000ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110399(ERAL1 antibody) at dilution of 1:50 (under 10x lens)
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Background
ERAL1 represents Era G-protein-like 1 (E. coli)
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References
- Dennerlein S, Rozanska A, Wydro M, Chrzanowska-Lightowlers ZM, Lightowlers RN. Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. The Biochemical journal. 430(3):551-8. 2010.
- He J, Cooper HM, Reyes A. Human C4orf14 interacts with the mitochondrial nucleoid and is involved in the biogenesis of the small mitochondrial ribosomal subunit. Nucleic acids research. 40(13):6097-108. 2012.
- He J, Cooper HM, Reyes A. Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis. Nucleic acids research. 40(13):6109-21. 2012.
- Dalla Rosa I, Durigon R, Pearce SF. MPV17L2 is required for ribosome assembly in mitochondria. Nucleic acids research. 42(13):8500-15. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"