Mouse EphB4/Eph Receptor B4 (His Tag) recombinant protein

Mouse EphB4/Eph Receptor B4 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

Ephrin type-B receptor 4

TYRO11; HTK; EPHB4; MYK1; MDK2; AI042935

P54761

Ephb4; Htk; Mdk2; Myk1

Human Cells

A DNA sequence encoding the mouse EPHB4 (P54761-1) extracellular domain (Met 1-Ala 539) was fused with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse EPHB4 comprises 535 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, the apparent molecular mass of His tagged EPHB4 monomer is approximately 70 kDa in SDS-PAGE under reducing conditions.

> 98 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse EphB4 at 2 μg/ml (100 μl/well) can bind mouse EphrinB2 with a linear range of 0.2-25 ng/ml.