Rat EphA7/Eph Receptor A7 (Fc Tag) recombinant protein

Rat EphA7/Eph Receptor A7 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7.

Ephrin type-A receptor 7

EHK3; MDK1; RP23-33D17.1; EK11; HEK11; EHK-3; EPHA7; CEK11; EBK

P54759

Epha7; Ehk-3; Ehk3

Human Cells

A DNA sequence encoding the rat Epha7 (NP_599158.1) (Met1-Ser539) was expressed with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat Epha7 consists 523 amino acids and predicts a molecular mass of 58.9 kDa.

> 90 % as determined by SDS-PAGE.

Measured by its binding ability in a functional ELISA.
Immobilized mouse EFNA4-His(Cat:50595-M08) at 10 μg/ml (100 μl/well) can bind rat EPHA7-Fc3, The EC₅₀ of rat EPHA7-Fc3 is 10-30 ng/mL.