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Product Name
EPB41 Polyclonal Antibody
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Description
Polyclonal antibody to EPB41
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
EPB41 antibody; 4.1R antibody; EL1 antibody; HE antibody; protein 4.1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 210-500 of human EPB41 (NP_001159478.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - EPB41 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EPB41 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - EPB41 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using EPB41 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - EPB41 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using EPB41 antibody at dilution of 1:200 (40x lens).
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Background
Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"