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Product Name
EPAC1 antibody
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Description
EPAC1 Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in CHO cells. Positive IHC detected in human kidney tissue, human heart tissue, human skeletal muscle tissue, human thyroid tissue. Positive IF detected in A431 cells. Observed molecular weight by Western-blot: 104 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat, hamster; other species not tested.
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Alternative names
CAMP GEFI antibody; CGEF1 antibody; EPAC antibody; EPAC 1 antibody; EPAC1 antibody; HSU79275 antibody; RAPGEF3 antibody; EPAC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EPAC1 recombinant protein (Accession Number: NM_001098531). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
CHO cells were subjected to SDS PAGE followed by western blot with Catalog No:110340(RAPGEF3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110340(RAPGEF3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110340(RAPGEF3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-RAPGEF3 (IP:Catalog No:110340, 4ug; Detection:Catalog No:110340 1:600) with mouse kidney tissue lysate 4800ug.
Immunofluorescent analysis of A431 cells using Catalog No:110340(RAPGEF3 Antibody) at dilution of 1:25 and FITC-Goat anti-Rabbit IgG
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Background
RAPGEF3 represents Rap guanine nucleotide exchange factor (GEF) 3
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