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Product Name
ENOX2 antibody
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Description
ENOX2 Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in MCF-7 cells, HeLa cells, mouse ovary tissue, PC-3 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
APK1 antibody; APK1 antigen antibody; COVA1 antibody; ENOX2 antibody; tNOX antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ENOX2 recombinant protein (Accession Number: BC019254). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110321(ENOX2 Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110321(ENOX2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ENOX2 (IP:Catalog No:110321, 3ug; Detection:Catalog No:110321 1:700) with MCF-7 cells lysate 4000ug.
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Background
Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock
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References
- Liu SC, Yang JJ, Shao KN, Chueh PJ. RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac. Biochemical and biophysical research communications. 365(4):672-7. 2008.
- Zeng ZM, Chuang SM, Chang TC. Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells. Experimental cell research. 318(14):1759-66. 2012.
- Su YC, Lin YH, Zeng ZM, Shao KN, Chueh PJ. Chemotherapeutic agents enhance cell migration and epithelial-to-mesenchymal transition through transient up-regulation of tNOX (ENOX2) protein. Biochimica et biophysica acta. 1820(11):1744-52. 2012.
- Tikhomirov AS, Shchekotikhin AE, Lee YH. Synthesis and Characterization of 4,11-Diaminoanthra[2,3-b]furan-5,10-diones: Tumor Cell Apoptosis through tNOX-Modulated NAD(+)/NADH Ratio and SIRT1. Journal of medicinal chemistry. 58(24):9522-34. 2015.
- Wang HM, Chuang SM, Su YC, Li YH, Chueh PJ. Down-regulation of tumor-associated NADH oxidase, tNOX (ENOX2), enhances capsaicin-induced inhibition of gastric cancer cell growth. Cell biochemistry and biophysics. 61(2):355-66. 2011.
- Liu NC, Hsieh PF, Hsieh MK. Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo. Journal of agricultural and food chemistry. 60(10):2758-65. 2012.
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